1NEM

Saccharide-RNA recognition in the neomycin B / RNA aptamer complex

Summary for 1NEM

• Entry DOI: 10.2210/pdb1nem/pdb
• Descriptor: 5'-R(*GP*GP*AP*CP*UP*GP*GP*GP*CP*GP*AP*GP*AP*AP*GP*UP*UP*UP*AP*GP*UP*CP*C)-3', 2,6-diamino-2,6-dideoxy-beta-L-idopyranose-(1-3)-beta-D-ribofuranose, 2,6-diamino-2,6-dideoxy-alpha-D-glucopyranose, ... (4 entities in total)
• Functional Keywords: rna aptamer, aminoglycoside, antibiotic, rna
• Total number of polymer chains: 1
• Total formula weight: 8111.16

Authors

Jiang, L.,Majumdar, A.,Hu, W.,Jaishree, T.J.,Xu, W.,Patel, D.J. (deposition date: 1999-03-15, release date: 1999-08-31, Last modification date: 2023-12-27)

Primary citation

Jiang, L.,Majumdar, A.,Hu, W.,Jaishree, T.J.,Xu, W.,Patel, D.J.
Saccharide-RNA recognition in a complex formed between neomycin B and an RNA aptamer
Structure Fold.Des., 7:817-827, 1999

PubMed Abstract: Aminoglycoside antibiotics can target RNA folds with micromolar affinity and inhibit biological processes ranging from protein biosynthesis to ribozyme action and viral replication. Specific features of aminoglycoside antibiotic-RNA recognition have been probed using chemical, biochemical, spectroscopic and computational approaches on both natural RNA targets and RNA aptamers identified through in vitro selection. Our previous studies on tobramycin-RNA aptamer complexes are extended to neomycin B bound to its selected RNA aptamer with 100 nM affinity.

PubMed: 10425683
DOI: 10.1016/S0969-2126(99)80105-1

Experimental method

SOLUTION NMR