1NEK

Complex II (Succinate Dehydrogenase) From E. Coli with ubiquinone bound

1NEK の概要

• エントリーDOI: 10.2210/pdb1nek/pdb
• 関連するPDBエントリー: 1nen
• 分子名称: Succinate dehydrogenase flavoprotein subunit, FE3-S4 CLUSTER, PROTOPORPHYRIN IX CONTAINING FE, ... (15 entities in total)
• 機能のキーワード: membrane protein, oxygen respiratory chain, oxidoreductase-electron transport complex, oxidoreductase/electron transport
• 由来する生物種: Escherichia coli; 詳細
• 細胞内の位置: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side: P0AC41; Cell inner membrane; Peripheral membrane protein: P07014; Cell inner membrane; Multi-pass membrane protein: P69054 P0AC44
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 123107.58

構造登録者

Yankovskaya, V.,Horsefield, R.,Tornroth, S.,Luna-Chavez, C.,Miyoshi, H.,Leger, C.,Byrne, B.,Cecchini, G.,Iwata, S. (登録日: 2002-12-11, 公開日: 2003-02-25, 最終更新日: 2024-11-20)

主引用文献

Yankovskaya, V.,Horsefield, R.,Tornroth, S.,Luna-Chavez, C.,Miyoshi, H.,Leger, C.,Byrne, B.,Cecchini, G.,Iwata, S.
Architecture of succinate dehydrogenase and reactive oxygen species generation.
Science, 299:700-704, 2003

PubMed Abstract: The structure of Escherichia coli succinate dehydrogenase (SQR), analogous to the mitochondrial respiratory complex II, has been determined, revealing the electron transport pathway from the electron donor, succinate, to the terminal electron acceptor, ubiquinone. It was found that the SQR redox centers are arranged in a manner that aids the prevention of reactive oxygen species (ROS) formation at the flavin adenine dinucleotide. This is likely to be the main reason SQR is expressed during aerobic respiration rather than the related enzyme fumarate reductase, which produces high levels of ROS. Furthermore, symptoms of genetic disorders associated with mitochondrial SQR mutations may be a result of ROS formation resulting from impaired electron transport in the enzyme.

PubMed: 12560550
DOI: 10.1126/science.1079605

実験手法

X-RAY DIFFRACTION (2.6 Å)