1N7D

Extracellular domain of the LDL receptor

1N7D の概要

• エントリーDOI: 10.2210/pdb1n7d/pdb
• 分子名称: Low-density lipoprotein receptor, alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: ldl-receptor, familial hypercholesterolemia, ldl, cholesterol metabolism, lipid transport
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 88314.84

構造登録者

Rudenko, G.,Henry, L.,Henderson, K.,Ichtchenko, K.,Brown, M.S.,Goldstein, J.L.,Deisenhofer, J. (登録日: 2002-11-13, 公開日: 2003-01-21, 最終更新日: 2025-11-12)

主引用文献

Rudenko, G.,Henry, L.,Henderson, K.,Ichtchenko, K.,Brown, M.S.,Goldstein, J.L.,Deisenhofer, J.
Structure of the LDL receptor extracellular domain at endosomal pH
Science, 298:2353-2358, 2002

PubMed Abstract: The low-density lipoprotein receptor mediates cholesterol homeostasis through endocytosis of lipoproteins. It discharges its ligand in the endosome at pH < 6. In the crystal structure at pH = 5.3, the ligand-binding domain (modules R2 to R7) folds back as an arc over the epidermal growth factor precursor homology domain (the modules A, B, beta propeller, and C). The modules R4 and R5, which are critical for lipoprotein binding, associate with the beta propeller via their calcium-binding loop. We propose a mechanism for lipoprotein release in the endosome whereby the beta propeller functions as an alternate substrate for the ligand-binding domain, binding in a calcium-dependent way and promoting lipoprotein release.

PubMed: 12459547
DOI: 10.1126/science.1078124

実験手法

X-RAY DIFFRACTION (3.7 Å)