1N35

lambda3 elongation complex with four phosphodiester bond formed

Summary for 1N35

• Entry DOI: 10.2210/pdb1n35/pdb
• Related: 1MUK 1MWH 1N1H 1N38
• Descriptor: 5'-R(P*GP*GP*GP*GP*G)-3', 5'-R(*AP*UP*UP*AP*GP*CP*CP*CP*CP*C)-3', Minor core protein lambda 3, ... (6 entities in total)
• Functional Keywords: rna polymerase, right hand configuration, bracelet, transferase-rna complex, transferase/rna
• Biological source: Mammalian orthoreovirus 3; More
• Cellular location: Virion (Potential): P17378
• Total number of polymer chains: 3
• Total formula weight: 148713.12

Authors

Tao, Y.,Farsetta, D.L.,Nibert, M.L.,Harrison, S.C. (deposition date: 2002-10-25, release date: 2002-12-25, Last modification date: 2024-04-03)

Primary citation

Tao, Y.,Farsetta, D.L.,Nibert, M.L.,Harrison, S.C.
RNA Synthesis in a Cage--Structural Studies of Reovirus Polymerase [lambda] 3
Cell(Cambridge,Mass.), 111:733-745, 2002

PubMed Abstract: The reovirus polymerase and those of other dsRNA viruses function within the confines of a protein capsid to transcribe the tightly packed dsRNA genome segments. The crystal structure of the reovirus polymerase, lambda3, determined at 2.5 A resolution, shows a fingers-palm-thumb core, similar to those of other viral polymerases, surrounded by major N- and C-terminal elaborations, which create a cage-like structure, with four channels leading to the catalytic site. This "caged" polymerase has allowed us to visualize the results of several rounds of RNA polymerization directly in the crystals. A 5' cap binding site on the surface of lambda3 suggests a template retention mechanism by which attachment of the 5' end of the plus-sense strand facilitates insertion of the 3' end of the minus-sense strand into the template channel.

PubMed: 12464184
DOI: 10.1016/S0092-8674(02)01110-8

Experimental method

X-RAY DIFFRACTION (2.5 Å)