1MYR

MYROSINASE FROM SINAPIS ALBA

Summary for 1MYR

• Entry DOI: 10.2210/pdb1myr/pdb
• Descriptor: MYROSINASE, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, beta-D-xylopyranose-(1-2)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-3)]2-acetamido-2-deoxy-beta-D-glucopyranose, ... (9 entities in total)
• Functional Keywords: family 1 glycosyl hydrolase, glucosinolate, myrosinase, tim barrel, glycosidase
• Biological source: Sinapis alba (white mustard)
• Cellular location: Vacuole: P29736
• Total number of polymer chains: 1
• Total formula weight: 62289.19

Authors

Burmeister, W.P.,Iori, R.,Palmieri, S.,Henrissat, B. (deposition date: 1997-03-23, release date: 1997-06-16, Last modification date: 2024-10-23)

Primary citation

Burmeister, W.P.,Cottaz, S.,Driguez, H.,Iori, R.,Palmieri, S.,Henrissat, B.
The crystal structures of Sinapis alba myrosinase and a covalent glycosyl-enzyme intermediate provide insights into the substrate recognition and active-site machinery of an S-glycosidase.
Structure, 5:663-675, 1997

PubMed Abstract: Myrosinase is the enzyme responsible for the hydrolysis of a variety of plant anionic 1-thio-beta-D-glucosides called glucosinolates. Myrosinase and glucosinolates, which are stored in different tissues of the plant, are mixed during mastication generating toxic by-products that are believed to play a role in the plant defence system. Whilst O-glycosidases are extremely widespread in nature, myrosinase is the only known S-glycosidase. This intriguing enzyme, which shows sequence similarities with O-glycosidases, offers the opportunity to analyze the similarities and differences between enzymes hydrolyzing S- and O-glycosidic bonds.

PubMed: 9195886
DOI: 10.1016/S0969-2126(97)00221-9

Experimental method

X-RAY DIFFRACTION (1.64 Å)