1MWE

THE X-RAY STRUCTURE OF A COMPLEX OF TERN N9 INFLUENZA VIRUS NEURAMINIDASE COMPLEXED WITH SIALIC ACID AT 4 DEGREES C REVEALING A SECOND SIALIC ACID BINDING SITE

Summary for 1MWE

• Entry DOI: 10.2210/pdb1mwe/pdb
• Descriptor: NEURAMINIDASE, alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
• Functional Keywords: hydrolase, neuraminidase, avian influenza, sialic acid binding, hemagglutination site, heme absorbing site
• Biological source: Influenza A virus
• Cellular location: Virion membrane (By similarity): P03472
• Total number of polymer chains: 1
• Total formula weight: 46645.48

Authors

Varghese, J.N. (deposition date: 1997-08-27, release date: 1998-03-04, Last modification date: 2024-11-13)

Primary citation

Varghese, J.N.,Colman, P.M.,van Donkelaar, A.,Blick, T.J.,Sahasrabudhe, A.,McKimm-Breschkin, J.L.
Structural evidence for a second sialic acid binding site in avian influenza virus neuraminidases.
Proc.Natl.Acad.Sci.USA, 94:11808-11812, 1997

PubMed Abstract: The x-ray structure of a complex of sialic acid (Neu5Ac) with neuraminidase N9 subtype from A/tern/Australia/G70C/75 influenza virus at 4 degrees C has revealed the location of a second Neu5Ac binding site on the surface of the enzyme. At 18 degrees C, only the enzyme active site contains bound Neu5Ac. Neu5Ac binds in the second site in the chair conformation in a similar way to which it binds to hemagglutinin. The residues that interact with Neu5Ac at this second site are mostly conserved in avian strains, but not in human and swine strains, indicating that it has some as-yet-unknown biological function in birds.

PubMed: 9342319
DOI: 10.1073/pnas.94.22.11808

Experimental method

X-RAY DIFFRACTION (1.7 Å)