Summary for 4DM0
| Entry DOI | 10.2210/pdb4dm0/pdb |
| Related | 1MUH 1MUS |
| Descriptor | Transposase for transposon Tn5, DNA TRANSFERRED STRAND, DNA NON-TRANSFERRED STRAND, ... (6 entities in total) |
| Functional Keywords | transposase, ribonuclease h-like motif, protein-dna complex, synaptic complex, dna recombination-dna complex, hydrolase-dna complex, hydrolase/dna |
| Biological source | Escherichia coli More |
| Total number of polymer chains | 3 |
| Total formula weight | 66173.18 |
| Authors | Klenchin, V.A.,Lovell, S.,Goryshin, I.Y.,Reznikoff, W.R.,Rayment, I. (deposition date: 2012-02-06, release date: 2012-02-22, Last modification date: 2023-09-13) |
| Primary citation | Lovell, S.,Goryshin, I.Y.,Reznikoff, W.R.,Rayment, I. Two-metal active site binding of a TN5 transposase synaptic complex Nat.Struct.Biol., 9:278-281, 2002 Cited by PubMed Abstract: A synaptic complex of Tn5 transposase with an extended outside end DNA duplex was prepared and crystallized, and its crystal structure was determined in an effort to reveal the role of metal ions in catalysis. Two Mn2+ ions bound to the active site when a single nucleotide of donor DNA was added to the 3' end of the transferred strand. Marked conformational changes were observed in the DNA bases closest to the active site. The position of the metal ions and the conformational changes of the DNA provide insight into the mechanism of hairpin formation and cleavage, and is consistent with a two-metal model for catalysis. PubMed: 11896402PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.5 Å) |
Structure validation
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