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4DM0

TN5 transposase: 20MER OUTSIDE END 2 MN complex

Replaces:  1MURReplaces:  1L1A
Entity
Entity IDChain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
1A
(A)
Transposase for transposon Tn5polymer47753367.11UniProt (Q46731)
Pfam (PF14706)
Pfam (PF01609)
Escherichia coliTnp
2B
(B)
DNA TRANSFERRED STRANDpolymer216526.21
3C
(C)
DNA NON-TRANSFERRED STRANDpolymer206052.91
4D, E, F
(A)
MANGANESE (II) IONnon-polymer54.93Chemie (MN)
5G
(A)
1,2-ETHANEDIOLnon-polymer62.11Chemie (EDO)
6H, I, J
(A, B, C)
waterwater18.0362Chemie (HOH)
Sequence modifications
A: 1 - 476 (UniProt: Q46731)
PDBExternal DatabaseDetails
Lys 54Glu 54engineered mutation
Ala 56Met 56engineered mutation
Lys 345Glu 345engineered mutation
Pro 372Leu 372engineered mutation
Gly 477-expression tag
Sequence viewer
Contents of the asymmetric unit
PolymersNumber of chains3
Total formula weight65946.3
Non-Polymers*Number of molecules4
Total formula weight226.9
All*Total formula weight66173.2
*Water molecules are not included.

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PDB entries from 2025-06-11

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