1MTL

Non-productive MUG-DNA complex

1MTL の概要

• エントリーDOI: 10.2210/pdb1mtl/pdb
• 関連するPDBエントリー: 1MUG
• 分子名称: 5'-D(*CP*GP*CP*GP*AP*GP*(AAB)P*TP*CP*GP*CP*G)-3', G/U mismatch-specific DNA glycosylase (3 entities in total)
• 機能のキーワード: glycosylase, cis-platin, inter-strand, non-productive, hydrolase-dna complex, hydrolase/dna
• 由来する生物種: Escherichia coli
• 細胞内の位置: Cytoplasm : P0A9H1
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 44535.29

構造登録者

Barrett, T.E.,Savva, R.,Barlow, T.,Brown, T.,Jiricny, J.,Pearl, L.H. (登録日: 2002-09-21, 公開日: 2002-09-27, 最終更新日: 2024-04-03)

主引用文献

Barrett, T.E.,Savva, R.,Barlow, T.,Brown, T.,Jiricny, J.,Pearl, L.H.
Structure of a DNA base-excision product resembling a cisplatin inter-strand adduct.
Nat.Struct.Biol., 5:697-701, 1998

PubMed Abstract: Base-excision of a self-complementary oligonucleotide with central G:T mismatches by the G:T/U-specific mismatch DNA glycosylase (MUG), generates an unusual DNA structure which is remarkably similar in conformation to an interstrand DNA adduct of the anti-tumor drug cis-diamminedichloroplatinum. The abasic sugars generated by excision of the mismatched thymines are extruded from the double-helix, and the 'widowed' deoxyguanosines rotate so that their N7 and O6 groups protrude into the minor groove of the duplex and restack in an interleaved intercalative geometry, generating a kink in the helix axis.

PubMed: 9699633
DOI: 10.1038/1394

実験手法

X-RAY DIFFRACTION (2.8 Å)