1MP9

TBP from a mesothermophilic archaeon, Sulfolobus acidocaldarius

Summary for 1MP9

• Entry DOI: 10.2210/pdb1mp9/pdb
• Descriptor: TATA-binding protein (2 entities in total)
• Functional Keywords: transcription regulation, dna-binding protein, transcription factor, dna binding protein
• Biological source: Sulfolobus acidocaldarius
• Total number of polymer chains: 2
• Total formula weight: 44996.66

Authors

Koike, H.,Kawashima-Ohya, Y.,Yamasaki, T.,Clowney, L.,Katsuya, Y.,Suzuki, M. (deposition date: 2002-09-12, release date: 2003-11-04, Last modification date: 2024-03-13)

Primary citation

Koike, H.,Kawashima-Ohya, Y.,Yamasaki, T.,Clowney, L.,Katsuya, Y.,Suzuki, M.
Origins of Protein Stability Revealed by Comparing Crystal Structures of TATA Binding Proteins.
Structure, 12:157-168, 2004

PubMed Abstract: The crystal structure of TATA binding protein (TBP) from a mesothermophilic archaeon, Sulfolobus acidocaldarius, has been determined at a resolution of 2.0 A with an R factor of 20.9%. By comparing this structure with the structures of TBPs from a hyperthermophilic archaeon and mesophilic eukaryotes, as well as by comparing amino acid sequences of TBPs from archaea, covering a wide range of optimum growth temperatures, two significant determinants of the stability of TBP have been identified: increasing the interior hydrophobicity by interaction between three residues, Val, Leu, and Ile, with further differentiation of the surface, and increasing its hydrophilicity and raising the cost of unfolding. These findings suggest directions along which the stability of TBP can be engineered.

PubMed: 14725775
DOI: 10.1016/j.str.2003.12.003

Experimental method

X-RAY DIFFRACTION (2 Å)