1ML3

Evidences for a flip-flop catalytic mechanism of Trypanosoma cruzi glyceraldehyde-3-phosphate dehydrogenase, from its crystal structure in complex with reacted irreversible inhibitor 2-(2-phosphono-ethyl)-acrylic acid 4-nitro-phenyl ester

1ML3 の概要

• エントリーDOI: 10.2210/pdb1ml3/pdb
• 関連するPDBエントリー: 1A7K 1GYP
• 分子名称: Glyceraldehyde 3-phosphate dehydrogenase, glycosomal, NICOTINAMIDE-ADENINE-DINUCLEOTIDE, (3-FORMYL-BUT-3-ENYL)-PHOSPHONIC ACID, ... (4 entities in total)
• 機能のキーワード: protein covalent-inhibitor complex, oxidoreductase
• 由来する生物種: Trypanosoma cruzi
• 細胞内の位置: Glycosome: P22513
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 158932.72

構造登録者

Castilho, M.S.,Pavao, F.,Oliva, G. (登録日: 2002-08-29, 公開日: 2003-07-08, 最終更新日: 2024-04-03)

• 主引用文献: Castilho, M.S.,Pavao, F.,Oliva, G.,Ladame, S.,Willson, M.,Perie, J.; Evidence for the Two Phosphate Binding Sites of an Analogue of the Thioacyl Intermediate for the Trypanosoma cruzi Glyceraldehyde-3-phosphate Dehydrogenase-Catalyzed Reaction, from Its Crystal Structure.; Biochemistry, 42:7143-7151, 2003; PubMed: 12795610; DOI: 10.1021/bi0206107

実験手法

X-RAY DIFFRACTION (2.5 Å)