1MJQ

METHIONINE REPRESSOR MUTANT (Q44K) PLUS COREPRESSOR (S-ADENOSYL METHIONINE) COMPLEXED TO AN ALTERED MET CONSENSUS OPERATOR SEQUENCE

Summary for 1MJQ

• Entry DOI: 10.2210/pdb1mjq/pdb
• Related: 1MJ2 1MJM 1MJO 1MJP
• Descriptor: MUTATED MET CONSENSUS OPERATOR DUPLEX, METHIONINE REPRESSOR, S-ADENOSYLMETHIONINE, ... (4 entities in total)
• Functional Keywords: transcription regulation, metj, methionine repressor, sheet-helix-helix, s-adenosyl methionine, dna, complex (transcription regulation-dna), transcription-dna complex, transcription/dna
• Biological source: Escherichia coli; More
• Cellular location: Cytoplasm: P0A8U6
• Total number of polymer chains: 12
• Total formula weight: 122687.58

Authors

Garvie, C.W.,Phillips, S.E.V. (deposition date: 1998-01-30, release date: 1999-08-02, Last modification date: 2023-08-02)

Primary citation

Garvie, C.W.,Phillips, S.E.
Direct and indirect readout in mutant Met repressor-operator complexes.
Structure Fold.Des., 8:905-914, 2000

PubMed Abstract: The methionine repressor, MetJ, represses the transcription of genes involved in methionine biosynthesis by binding to arrays of two to five adjacent copies of an eight base-pair 'metbox' sequence. Naturally occurring operators differ from the consensus sequence to a greater extent as the number of metboxes increases. MetJ, while accommodating this sequence variation in natural operators, is very sensitive to particular base changes, even where bases are not directly contacted in the crystal structure of a complex formed between the repressor and consensus operator.

PubMed: 10986458
DOI: 10.1016/S0969-2126(00)00182-9

Experimental method

X-RAY DIFFRACTION (2.4 Å)