1MIS

STRUCTURE OF RNA (5'-R(GP*CP*GP*GP*AP*CP*GP*C)-3') ANTI-PARALLEL RNA DUPLEX WITH TANDEM G:A MISMATCHES, NMR, MINIMIZED AVERAGE STRUCTURE

Summary for 1MIS

• Entry DOI: 10.2210/pdb1mis/pdb
• Descriptor: RNA (5'-R(*GP*CP*GP*GP*AP*CP*GP*C)-3') (1 entity in total)
• Functional Keywords: rna, tandem g:a mismatch, ribonucleic acid
• Total number of polymer chains: 2
• Total formula weight: 5161.23

Authors

Wu, M.,Turner, D.H. (deposition date: 1997-03-26, release date: 1997-07-07, Last modification date: 2024-05-22)

Primary citation

Wu, M.,Turner, D.H.
Solution structure of (rGCGGACGC)2 by two-dimensional NMR and the iterative relaxation matrix approach.
Biochemistry, 35:9677-9689, 1996

PubMed Abstract: The three-dimensional solution structure of the RNA self-complementary duplex [sequence: see text] was derived from two-dimensional NMR and the iterative relaxation matrix approach. Each GA mismatch forms two hydrogen bonds: A-NH6 to G-O6 and A-N1 to G-NH1 (imino). This is the first three-dimensional RNA structure with imino hydrogen-bonded tandem GA mismatches. This GA structure is totally different from the sheared tandem GA structure in [sequence: see text] which also has two hydrogen bonds: A-N7 to G-NH2 and A-NH6 to G-N3 [SantaLucia, J., Jr., & Turner, D. H. (1993) Biochemistry 32, 12612-12623]. In particular, the sheared and imino GA mismatches produce a narrowing and widening of the backbone, respectively. The results show that substitutions of Watson-Crick base pairs can have dramatic effects on the three-dimensional structures of adjacent non-Watson-Crick paired regions; i.e., the structure depends on sequence context. Thus compensating substitutions in site-directed mutagenesis experiments may not always restore biological activities.

PubMed: 8703939
DOI: 10.1021/bi960133q

Experimental method

SOLUTION NMR