1MIJ
Crystal Structure of the Homeo-prospero Domain of D. melanogaster Prospero
Summary for 1MIJ
| Entry DOI | 10.2210/pdb1mij/pdb |
| Descriptor | Protein prospero (2 entities in total) |
| Functional Keywords | homeodomain, dna-binding domain, prospero domain, 4-helix bundle, transcription |
| Biological source | Drosophila melanogaster (fruit fly) |
| Cellular location | Nucleus: P29617 |
| Total number of polymer chains | 1 |
| Total formula weight | 18454.40 |
| Authors | Ryter, J.M.,Doe, C.Q.,Matthews, B.W. (deposition date: 2002-08-23, release date: 2002-12-04, Last modification date: 2024-11-06) |
| Primary citation | Ryter, J.M.,Doe, C.Q.,Matthews, B.W. Structure of the DNA Binding Region of Prospero Reveals a Novel Homeo-Prospero Domain Structure, 10:1541-1549, 2002 Cited by PubMed Abstract: The Prospero transcription factor promotes neural differentiation in Drosophila, and its activity is tightly regulated by modulating its subcellular localization. Prospero is exported from the nucleus of neural precursors but imported into the nucleus of daughter cells, which is necessary for their proper differentiation. Prospero has a highly divergent putative homeodomain adjacent to a conserved Prospero domain; both are required for sequence-specific DNA binding. Here we show that the structure of these two regions consists of a single structural unit (a homeo-prospero domain), in which the Prospero domain region is in position to contribute to DNA binding and also to mask a defined nuclear export signal that is within the putative homeodomain region. We propose that the homeo-prospero domain coordinately regulates Prospero nuclear localization and DNA binding specificity. PubMed: 12429095DOI: 10.1016/S0969-2126(02)00883-3 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.05 Å) |
Structure validation
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