1MEY

CRYSTAL STRUCTURE OF A DESIGNED ZINC FINGER PROTEIN BOUND TO DNA

Summary for 1MEY

• Entry DOI: 10.2210/pdb1mey/pdb
• Descriptor: DNA (5'-D(*AP*TP*GP*AP*GP*GP*CP*AP*GP*AP*AP*CP*T)-3'), DNA (5'-D(*TP*AP*GP*TP*TP*CP*TP*GP*CP*CP*TP*(C38)P*A)-3'), CONSENSUS ZINC FINGER, ... (6 entities in total)
• Functional Keywords: zinc finger, protein-dna interaction, protein design, complex (zinc finger-dna), transferase-dna complex, transferase/dna
• Total number of polymer chains: 7
• Total formula weight: 47137.17

Authors

Kim, C.A.,Berg, J.M. (deposition date: 1996-09-27, release date: 1997-03-12, Last modification date: 2024-02-14)

Primary citation

Kim, C.A.,Berg, J.M.
A 2.2 A Resolution Crystal Structure of a Designed Zinc Finger Protein Bound to DNA
Nat.Struct.Biol., 3:940-945, 1996

PubMed Abstract: Considerable recent effort has been devoted to the design and selection of sequence-specific DNA binding proteins based on tandem arrays of Cys2His2 zinc finger domains. While the DNA binding properties of these designed proteins have been studied extensively, the structural basis for site-specific binding has not been examined experimentally. Here we report the crystal structure of a complex between a protein comprised of three consensus-sequence-based zinc finger domains and an oligonucleotide corresponding to a favourable DNA binding site. This structure reveals relatively simple modular interactions and structural adaptations that compensate for differences in contact residue side-chain lengths.

PubMed: 8901872
DOI: 10.1038/nsb1196-940

Experimental method

X-RAY DIFFRACTION (2.2 Å)