1MDX

Crystal structure of ArnB transferase with pyridoxal 5' phosphate

1MDX の概要

• エントリーDOI: 10.2210/pdb1mdx/pdb
• 関連するPDBエントリー: 1MDO 1MDZ
• 分子名称: UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase, 2-OXOGLUTARIC ACID, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: type 1 aminotransferase fold, transferase
• 由来する生物種: Salmonella enterica subsp. enterica serovar Typhimurium
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 43390.19

構造登録者

Noland, B.W.,Newman, J.M.,Hendle, J.,Badger, J.,Christopher, J.A.,Tresser, J.,Buchanan, M.D.,Wright, T.A.,Rutter, M.E.,Sanderson, W.E.,Muller-Dieckmann, H.-J.,Gajiwala, K.S.,Sauder, J.M.,Buchanan, S.G. (登録日: 2002-08-07, 公開日: 2002-12-11, 最終更新日: 2024-12-25)

主引用文献

Noland, B.W.,Newman, J.M.,Hendle, J.,Badger, J.,Christopher, J.A.,Tresser, J.,Buchanan, M.D.,Wright, T.,Rutter, M.E.,Sanderson, W.E.,Muller-Dieckmann, H.-J.,Gajiwala, K.,Buchanan, S.G.
Structural studies of Salmonella typhimurium ArnB (PmrH) aminotransferase: A 4-amino-4-deoxy-L-arabinose lipopolysaccharide modifying enzyme
Structure, 10:1569-1580, 2002

PubMed Abstract: Lipid A modification with 4-amino-4-deoxy-L-arabinose confers on certain pathogenic bacteria, such as Salmonella, resistance to cationic antimicrobial peptides, including those derived from the innate immune system. ArnB catalysis of amino group transfer from glutamic acid to the 4"-position of a UDP-linked ketopyranose molecule to form UDP-4-amino-4-deoxy-L-arabinose represents a key step in the lipid A modification pathway. Structural and functional studies of the ArnB aminotransferase were undertaken by combining X-ray crystallography with biochemical analyses. High-resolution crystal structures were solved for two native forms and one covalently inhibited form of S. typhimurium ArnB. These structures permitted identification of key residues involved in substrate binding and catalysis, including a rarely observed nonprolyl cis peptide bond in the active site.

PubMed: 12429098
DOI: 10.1016/S0969-2126(02)00879-1

実験手法

X-RAY DIFFRACTION (1.96 Å)