1MCW

THREE-DIMENSIONAL STRUCTURE OF A HYBRID LIGHT CHAIN DIMER. PROTEIN ENGINEERING OF A BINDING CAVITY

1MCW の概要

• エントリーDOI: 10.2210/pdb1mcw/pdb
• 分子名称: IMMUNOGLOBULIN WEIR (LIGHT CHAIN), IMMUNOGLOBULIN MCG (LIGHT CHAIN) (2 entities in total)
• 機能のキーワード: immunoglobulin
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 45744.48

構造登録者

Ely, K.R.,Herron, J.N.,Edmundson, A.B. (登録日: 1989-05-09, 公開日: 1990-10-15, 最終更新日: 2024-10-16)

主引用文献

Ely, K.R.,Herron, J.N.,Edmundson, A.B.
Three-dimensional structure of a hybrid light chain dimer: protein engineering of a binding cavity.
Mol.Immunol., 27:101-114, 1990

PubMed Abstract: An attempt was made to engineer a binding site and check its structure by X-ray analysis. Two human light chains (Mcg and Weir), with "variable" domain sequences differing in 36 positions, were hybridized into a heterologous dimer and crystallized in ammonium sulfate by the same procedure used for the trigonal form of the Mcg dimer. The three-dimensional structure of the hybrid was determined at 3.5-A resolution by difference Fourier analysis, interactive model building with computer graphics and crystallographic refinement. In the heterologous dimer, the Weir protein behaved as the structural analog of the heavy chain in an antigen binding fragment, while the Mcg protein assumed the role of the light chain component. The hybrid and the Mcg dimer were closely similar in overall structure, an observation probably correlated with the deliberate cleavage of the intrachain disulfide bond in the variable domain of the Weir protein during the hybridization procedure. Examination of the crystal structure of the hybrid suggested that the cleavage resulted in the relaxation of restraints which might otherwise have interfered with the formation of an Mcg-like dimer. There were six substitutions among the residues lining the binding cavities of the hybrid and Mcg dimer. These substitutions significantly affected the sizes, shapes and binding properties of the two cavities.

PubMed: 2108322
DOI: 10.1016/0161-5890(90)90105-9

実験手法

X-RAY DIFFRACTION (3.5 Å)