1MA8

A-DNA decamer GCGTA(UMS)ACGC with incorporated 2'-methylseleno-uridine

Summary for 1MA8

• Entry DOI: 10.2210/pdb1ma8/pdb
• Descriptor: 5'-D(*GP*CP*GP*TP*AP*UMSP*AP*CP*GP*C)-3' (2 entities in total)
• Functional Keywords: 2'-methylseleno-uridine, dna
• Total number of polymer chains: 2
• Total formula weight: 6247.93

Authors

Teplova, M.,Wilds, C.J.,Wawrzak, Z.,Tereshko, V.,Du, Q.,Carrasco, N.,Huang, Z.,Egli, M. (deposition date: 2002-08-01, release date: 2002-12-11, Last modification date: 2024-02-14)

Primary citation

Teplova, M.,Wilds, C.J.,Wawrzak, Z.,Tereshko, V.,Du, Q.,Carrasco, N.,Huang, Z.,Egli, M.
Covalent incorporation of selenium into oligonucleotides for X-ray crystal structure determination via MAD: proof of principle
BIOCHIMIE, 84:849-858, 2002

PubMed Abstract: Selenium was incorporated into an oligodeoxynucleotide in the form of 2'-methylseleno-uridine (U(Se)). The X-ray crystal structure of the duplex left open bracket d(GCGTA)U(Se)d(ACGC) right open bracket (2) was determined by the multiwavelength anomalous dispersion (MAD) technique and refined to a resolution of 1.3 A, demonstrating that selenium can selectively substitute oxygen in DNA and that the resulting compounds are chemically stable. Since derivatization at the 2'-alpha-position with selenium does not affect the preference of the sugar for the C3'-endo conformation, this strategy is suitable for incorporating selenium into RNA. The availability of selenium-containing nucleic acids for crystallographic phasing offers an attractive alternative to the commonly used halogenated pyrimidines.

PubMed: 12458077
DOI: 10.1016/S0300-9084(02)01440-2

Experimental method

X-RAY DIFFRACTION (1.3 Å)