1M1F

Kid toxin protein from E.coli plasmid R1

1M1F の概要

• エントリーDOI: 10.2210/pdb1m1f/pdb
• 関連するPDBエントリー: 4VUB
• 分子名称: Kid toxin protein, PHOSPHATE ION (3 entities in total)
• 機能のキーワード: toxin-antitoxin, plasmid maintenance, post segregational killing, dna replication, mutational analysis, ccdb, toxin
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 23886.50

構造登録者

Hargreaves, D.,Santos-Sierra, S.,Giraldo, R.,Sabariegos-Jareno, R.,de la Cueva-Mendez, G.,Boelens, R.,Diaz-Orejas, R.,Rafferty, J.B. (登録日: 2002-06-19, 公開日: 2002-11-20, 最終更新日: 2024-02-14)

主引用文献

Hargreaves, D.,Santos-Sierra, S.,Giraldo, R.,Sabariegos-Jareno, R.,de la Cueva-Mendez, G.,Boelens, R.,Diaz-Orejas, R.,Rafferty, J.B.
Structural and Functional Analysis of the Kid toxin protein from E.coli plasmid R1
Structure, 10:1425-1433, 2002

PubMed Abstract: We have determined the structure of Kid toxin protein from E. coli plasmid R1 involved in stable plasmid inheritance by postsegregational killing of plasmid-less daughter cells. Kid forms a two-component system with its antagonist, Kis antitoxin. Our 1.4 A crystal structure of Kid reveals a 2-fold symmetric dimer that closely resembles the DNA gyrase-inhibitory toxin protein CcdB from E. coli F plasmid despite the lack of any notable sequence similarity. Analysis of nontoxic mutants of Kid suggests a target interaction interface associated with toxicity that is in marked contrast to that proposed for CcdB. A possible region for interaction of Kid with the antitoxin is proposed that overlaps with the target binding site and may explain the mode of antitoxin action.

PubMed: 12377128
DOI: 10.1016/S0969-2126(02)00856-0

実験手法

X-RAY DIFFRACTION (1.4 Å)