1LTI

HEAT-LABILE ENTEROTOXIN (LT-I) COMPLEX WITH T-ANTIGEN

Summary for 1LTI

• Entry DOI: 10.2210/pdb1lti/pdb
• Related PRD ID: PRD_900084
• Descriptor: HEAT LABILE ENTEROTOXIN TYPE I, beta-D-galactopyranose-(1-3)-2-acetamido-2-deoxy-alpha-D-galactopyranose, beta-D-galactopyranose, ... (6 entities in total)
• Functional Keywords: adp-ribosyl transferase, enterotoxin
• Biological source: Escherichia coli; More
• Total number of polymer chains: 7
• Total formula weight: 87831.12

Authors

Van Den Akker, F.,Hol, W.G.J. (deposition date: 1996-05-09, release date: 1996-08-17, Last modification date: 2024-11-20)

Primary citation

van den Akker, F.,Steensma, E.,Hol, W.G.
Tumor marker disaccharide D-Gal-beta 1, 3-GalNAc complexed to heat-labile enterotoxin from Escherichia coli.
Protein Sci., 5:1184-1188, 1996

PubMed Abstract: Heat-labile enterotoxin (LT) is part of the cholera toxin (CT) family and consists of a catalytic A subunit and a B pentamer that serves to recognize the oligosaccharide part of the GM1 ganglioside receptor. We report here the crystal structure of heat-labile enterotoxin in complex with the disaccharide portion of the Thomsen-Friedenreich (T-antigen) tumor marker. The toxin:carbohydrate complex is determined to 2.13 A resolution, yielding an R-factor of 18.5%. The T-antigen disaccharide, D-Gal-beta 1,3-GalNAc-Ser/Thr, is present in more than 85% of human carcinomas and monitoring its autoimmune response is used for the early detection of tumors. Insight into the molecular recognition of this tumor antigen by sugar binding proteins can benefit the development of a diagnostic tool for human carcinomas as well as a T-antigen directed anticancer drug delivery system.

PubMed: 8762150

Experimental method

X-RAY DIFFRACTION (2.13 Å)