1LPZ

CRYSTAL STRUCTURE OF FXA IN COMPLEX WITH 41.

1LPZ の概要

• エントリーDOI: 10.2210/pdb1lpz/pdb
• 関連するPDBエントリー: 1LPG 1LPK 1LQD 1LQE
• 分子名称: Blood coagulation factor Xa, CALCIUM ION, 1-(3-carbamimidoylbenzyl)-N-(3,5-dichlorobenzyl)-4-methyl-1H-indole-2-carboxamide, ... (5 entities in total)
• 機能のキーワード: protein inhibitor complex, blood coagulation factor, serine proteinase, hydrolase
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Secreted: P00742 P00742
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 44266.84

構造登録者

Schreuder, H.A.,Brachvogel, V.,Liesum, A. (登録日: 2002-05-08, 公開日: 2003-05-08, 最終更新日: 2011-07-13)

主引用文献

Matter, H.,Defossa, E.,Heinelt, U.,Blohm, P.M.,Schneider, D.,Mueller, A.,Herok, S.,Schreuder, H.A.,Liesum, A.,Brachvogel, V.,Loenze, P.,Walser, A.,Al-Obeidi, F.,Wildgoose, P.
Design and Quantitative Structure-Activity relationship of 3-amidinobenzyl-1H-indole-2-carboxamides as potent, nonchiral, and selective inhibitors of blood coagulation factor Xa
J.Biol.Chem., 45:2749-2769, 2002

PubMed Abstract: A series of 138 nonchiral 3-amidinobenzyl-1H-indole-2-carboxamides and analogues as inhibitors of the blood coagulation enzyme factor Xa (fXa) were designed, synthesized, and investigated by X-ray structure analysis and 3D quantitative structure-activity relationship (QSAR) studies (CoMFA, CoMSIA) in order to identify important protein-ligand interactions responsible for biological affinity and selectivity. Several compounds from this series are highly potent and selective inhibitors of this important enzyme linking extrinsic and intrinsic coagulation pathways. To rationalize biological affinity and to provide guidelines for further design, all compounds were docked into the factor Xa binding site. Those docking studies were based on X-ray structures of factor Xa in complex with literature-known inhibitors. It was possible to validate those binding modes by four X-ray crystal structures of representative ligands in factor Xa, while one ligand was additionally crystallized in trypsin to rationalize requirements for selective factor Xa inhibition. The 3D-QSAR models based on a superposition rule derived from these docking studies were validated using conventional and cross-validated r(2) values using the leave-one-out method and repeated analyses using two randomly chosen cross-validation groups plus randomization of biological activities. This led to consistent and highly predictive 3D-QSAR models with good correlation coefficients for both CoMFA and CoMSIA, which were found to correspond to experimentally determined factor Xa binding site topology in terms of steric, electrostatic, and hydrophobic complementarity. Subsets selected as smaller training sets using 2D fingerprints and maximum dissimilarity methods resulted in 3D-QSAR models with remarkable correlation coefficients and a high predictive power. The final quantitative SAR information agrees with all experimental data for the binding topology and thus provides reasonable activity predictions for novel factor Xa inhibitors.

PubMed: 12061878
DOI: 10.1021/jm0111346

実験手法

X-RAY DIFFRACTION (2.4 Å)