1LPW

Solution structure of the yeast spliceosomal U2 snRNA-intron branch site helix featuring a conserved pseudouridine

Summary for 1LPW

• Entry DOI: 10.2210/pdb1lpw/pdb
• Related: 1LMV
• NMR Information: BMRB: 5394
• Descriptor: 5'-R(*GP*GP*UP*GP*(PSU)P*AP*GP*UP*A)-3', 5'-R(*UP*AP*CP*UP*AP*AP*CP*AP*CP*C)-3' (2 entities in total)
• Functional Keywords: u2 snrna, branch site, pseudouridine, rna
• Biological source: Saccharomyces cerevisiae (baker's yeast); More
• Total number of polymer chains: 2
• Total formula weight: 6017.70

Authors

Newby, M.I.,Greenbaum, N.L. (deposition date: 2002-05-08, release date: 2002-11-27, Last modification date: 2024-05-22)

Primary citation

Newby, M.I.,Greenbaum, N.L.
Sculpting of the Spliceosomal Branch Site Recognition Motif by a Conserved Pseudouridine
Nat.Struct.Biol., 12:958-965, 2002

PubMed Abstract: Pairing of a consensus sequence of the precursor (pre)-mRNA intron with a short region of the U2 small nuclear (sn)RNA during assembly of the eukaryotic spliceosome results in formation of a complementary helix of seven base pairs with a single unpaired adenosine residue. The 2' OH of this adenosine, called the branch site, brings about nucleophilic attack at the pre-mRNA 5' splice site in the first step of splicing. Another feature of this pairing is the phylogenetic conservation of a pseudouridine (psi) residue in U2 snRNA nearly opposite the branch site. We show that the presence of this psi in the pre-mRNA branch-site helix of Saccharomyces cerevisiae induces a dramatically altered architectural landscape compared with that of its unmodified counterpart. The psi-induced structure places the nucleophile in an accessible position for the first step of splicing.

PubMed: 12426583
DOI: 10.1038/nsb873

Experimental method

SOLUTION NMR