1LNL

Structure of deoxygenated hemocyanin from Rapana thomasiana

1LNL の概要

• エントリーDOI: 10.2210/pdb1lnl/pdb
• 分子名称: hemocyanin, 2-acetamido-2-deoxy-beta-D-glucopyranose, COPPER (II) ION (3 entities in total)
• 機能のキーワード: hemocyanin, deoxygenated form, oxygen, metal binding site, cooperativity, oxygen storage-transport complex, oxygen storage/transport
• 由来する生物種: Rapana thomasiana
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 141607.95

構造登録者

Perbandt, M.,Guthoehrlein, E.W.,Rypniewski, W.,Idakieva, K.,Stoeva, S.,Voelter, W.,Genov, N.,Betzel, C. (登録日: 2002-05-03, 公開日: 2003-06-03, 最終更新日: 2024-10-16)

主引用文献

Perbandt, M.,Guthoehrlein, E.W.,Rypniewski, W.,Idakieva, K.,Stoeva, S.,Voelter, W.,Genov, N.,Betzel, C.
The structure of a functional unit from the wall of a gastropod hemocyanin offers a possible mechanism for cooperativity
Biochemistry, 42:6341-6346, 2003

PubMed Abstract: Structure-function relationships in a molluscan hemocyanin have been investigated by determining the crystal structure of the Rapana thomasiana (gastropod) hemocyanin functional unit RtH2e in deoxygenated form at 3.38 A resolution. This is the first X-ray structure of an unit from the wall of the molluscan hemocyanin cylinder. The crystal structure of RtH2e demonstrates molecular self-assembly of six identical molecules forming a regular hexameric cylinder. This suggests how the functional units are ordered in the wall of the native molluscan hemocyanins. The molecular arrangement is stabilized by specific protomer-to-protomer interactions, which are probably typical for the functional units building the wall of the cylinders. A molecular mechanism for cooperative dioxygen binding in molluscan hemocyanins is proposed on the basis of the molecular interactions between the protomers. In particular, the deoxygenated RtH2e structure reveals a tunnel leading from two opposite sides of the molecule to the active site. The tunnel represents a possible entrance pathway for dioxygen molecules. No such tunnels have been observed in the crystal structure of the oxy-Odg, a functional unit from the Octopus dofleini (cephalopod) hemocyanin in oxygenated form.

PubMed: 12767214
DOI: 10.1021/bi020672x

実験手法

X-RAY DIFFRACTION (3.3 Å)