1LJX

THE STRUCTURE OF D(TPGPCPGPCPA)2 AT 293K: COMPARISON OF THE EFFECT OF SEQUENCE AND TEMPERATURE

Summary for 1LJX

• Entry DOI: 10.2210/pdb1ljx/pdb
• Descriptor: 5'-D(*TP*GP*CP*GP*CP*A)-3', MAGNESIUM ION (3 entities in total)
• Functional Keywords: z-dna hexamer with at base pairs at the terminal, dna
• Total number of polymer chains: 2
• Total formula weight: 3667.05

Authors

Thiyagarajan, S.,Satheesh Kumar, P.,Rajan, S.S.,Gautham, N. (deposition date: 2002-04-23, release date: 2002-05-03, Last modification date: 2023-08-16)

Primary citation

Thiyagarajan, S.,Satheesh Kumar, P.,Rajan, S.S.,Gautham, N.
Structure of d(TGCGCA)2 at 293 K: comparison of the effects of sequence and temperature.
Acta Crystallogr.,Sect.D, 58:1381-1384, 2002

PubMed Abstract: The crystal structure of a hexameric DNA fragment with the sequence d(TGCGCA)(2) has been solved and refined at 293 K at a resolution of 1.64 A. The molecule adopts a left-handed Z-type helical conformation which is common in alternating pyrimidine-purine sequences. The presence of A.T base pairs at the two terminals does not perturb the structure to any great degree. However, several sequence-specific microstructural changes are noticeable. The structure of the identical sequence determined at 120 K involving somewhat different crystallization conditions has been reported previously [Harper et al. (1998), Acta Cryst. D54, 1273-1284]. A comparison of the present structure with that at low temperature and with that of d(CGCGCG)(2) shows that the effect of the change in sequence is greater than the combined effect of changes in temperature and environment.

PubMed: 12136163
DOI: 10.1107/S090744490200896X

Experimental method

X-RAY DIFFRACTION (1.64 Å)