1LJM

DNA recognition is mediated by conformational transition and by DNA bending

1LJM の概要

• エントリーDOI: 10.2210/pdb1ljm/pdb
• 分子名称: RUNX1 transcription factor, CHLORIDE ION (3 entities in total)
• 機能のキーワード: immunoglobulin fold, beta-sandwich, transcription
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus: Q01196
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 29336.61

構造登録者

Bartfeld, D.,Shimon, L.,Couture, G.C.,Rabinovich, D.,Frolow, F.,Levanon, D.,Groner, Y.,Shakked, Z. (登録日: 2002-04-22, 公開日: 2002-11-06, 最終更新日: 2023-08-16)

主引用文献

Bartfeld, D.,Shimon, L.,Couture, G.,Rabinovich, D.,Frolow, F.,Levanon, D.,Groner, Y.,Shakked, Z.
DNA Recognition by the RUNX1 Transcription Factor Is Mediated by an Allosteric Transition in the RUNT Domain and by DNA Bending.
Structure, 10:1395-1407,

PubMed Abstract: The Runt domain proteins are transcription regulators of major developmental pathways. Here we present the crystal structures of the Runt domain (RD) of the human protein RUNX1 and its DNA binding site in their free states and compare them with the published crystal structures of RD bound to DNA and to the partner protein CBFbeta. We demonstrate that (1) RD undergoes an allosteric transition upon DNA binding, which is further stabilized by CBFbeta, and that (2) the free DNA target adopts a bent-helical conformation compatible with that of the complex. These findings elucidate the mechanism by which CBFbeta enhances RD binding to DNA as well as the role of the intrinsic conformation of the DNA target in the recognition process.

PubMed: 12377125
DOI: 10.1016/S0969-2126(02)00853-5

実験手法

X-RAY DIFFRACTION (2.5 Å)