1LFD

CRYSTAL STRUCTURE OF THE ACTIVE RAS PROTEIN COMPLEXED WITH THE RAS-INTERACTING DOMAIN OF RALGDS

1LFD の概要

• エントリーDOI: 10.2210/pdb1lfd/pdb
• 分子名称: RALGDS, RAS, MAGNESIUM ION, ... (5 entities in total)
• 機能のキーワード: complex (ralgds-ras), ral, effector interaction, complex (ralgds-ras) complex, complex (ralgds/ras)
• 由来する生物種: Rattus norvegicus (Norway rat); 詳細
• 細胞内の位置: Cytoplasm : Q03386; Cell membrane. Isoform 2: Nucleus: P01112
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 59080.50

構造登録者

Huang, L.,Hofer, F.,Martin, G.S.,Kim, S.-H. (登録日: 1998-04-29, 公開日: 1999-05-04, 最終更新日: 2024-05-22)

主引用文献

Huang, L.,Hofer, F.,Martin, G.S.,Kim, S.H.
Structural basis for the interaction of Ras with RalGDS.
Nat.Struct.Biol., 5:422-426, 1998

PubMed Abstract: The Ras protein signals to a number of distinct pathways by interacting with diverse downstream effectors. Among the effectors of Ras are the Raf kinase and RalGDS, a guanine nucleotide dissociation stimulator specific for Ral. Despite the absence of significant sequence similarities, both effectors bind directly to Ras, but with different specificities. We report here the 2.1 A crystal structure of the complex between Ras and the Ras-interacting domain (RID) of RalGDS. This structure reveals that the beta-sheet of the RID joins the switch I region of Ras to form an extended beta-sheet with a topology similar to that found in the Rap-Raf complex. However, the side chain interactions at the joining junctions of the two interacting systems and the relative orientation of the two binding domains are distinctly different. Furthermore, in the case of the Ras-RID complex a second RID molecule also interacts with a different part of the Ras molecule, the switch II region. These findings account for the cross-talk between the Ras and Ral pathways and the specificity with which Ras distinguishes the two effectors.

PubMed: 9628477
DOI: 10.1038/nsb0698-422

実験手法

X-RAY DIFFRACTION (2.1 Å)