1LC6

Solution Structure of the U6 Intramolecular Stem-loop RNA

Summary for 1LC6

• Entry DOI: 10.2210/pdb1lc6/pdb
• NMR Information: BMRB: 5371
• Descriptor: U6 Intramolecular Stem-loop RNA (1 entity in total)
• Functional Keywords: rna, stem-loop, pentaloop
• Total number of polymer chains: 1
• Total formula weight: 7668.61

Authors

Huppler, A.,Nikstad, L.J.,Allmann, A.M.,Brow, D.A.,Butcher, S.E. (deposition date: 2002-04-05, release date: 2002-05-29, Last modification date: 2024-05-22)

Primary citation

Huppler, A.,Nikstad, L.J.,Allmann, A.M.,Brow, D.A.,Butcher, S.E.
Metal binding and base ionization in the U6 RNA intramolecular stem-loop structure.
Nat.Struct.Biol., 9:431-435, 2002

PubMed Abstract: U6 RNA is a key component of the catalytic core of the spliceosome. A metal ion essential for the first catalytic step of pre-mRNA splicing binds to the U80 Sp phosphate oxygen within the yeast U6 intramolecular stem-loop (ISL). Here we present the first structural data for U6 RNA, revealing the three-dimensional structure of the highly conserved U6 ISL. The ISL binds metal ion at the U80 site with the same stereo specificity as the intact spliceosome. The metal-binding site is adjacent to a readily protonated C.A wobble pair. Protonation of the C.A pair and metal binding are mutually antagonistic. These results support a ribozyme model for U6 RNA function and suggest a possible mechanism for the regulation of RNA splicing.

PubMed: 11992125
DOI: 10.1038/nsb800

Experimental method

SOLUTION NMR