1LBH

INTACT LACTOSE OPERON REPRESSOR WITH GRATUITOUS INDUCER IPTG

1LBH の概要

• エントリーDOI: 10.2210/pdb1lbh/pdb
• 分子名称: INTACT LACTOSE OPERON REPRESSOR WITH GRATUITOUS INDUCER IPTG, 1-methylethyl 1-thio-beta-D-galactopyranoside (2 entities in total)
• 機能のキーワード: transcription regulation, dna-binding, repressor
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 155581.41

構造登録者

Lewis, M.,Chang, G.,Horton, N.C.,Kercher, M.A.,Pace, H.C.,Lu, P. (登録日: 1996-02-17, 公開日: 1996-07-11, 最終更新日: 2024-02-14)

主引用文献

Lewis, M.,Chang, G.,Horton, N.C.,Kercher, M.A.,Pace, H.C.,Schumacher, M.A.,Brennan, R.G.,Lu, P.
Crystal structure of the lactose operon repressor and its complexes with DNA and inducer.
Science, 271:1247-1254, 1996

PubMed Abstract: The lac operon of Escherichia coli is the paradigm for gene regulation. Its key component is the lac repressor, a product of the lacI gene. The three-dimensional structures of the intact lac repressor, the lac repressor bound to the gratuitous inducer isopropyl-beta-D-1-thiogalactoside (IPTG) and the lac repressor complexed with a 21-base pair symmetric operator DNA have been determined. These three structures show the conformation of the molecule in both the induced and repressed states and provide a framework for understanding a wealth of biochemical and genetic information. The DNA sequence of the lac operon has three lac repressor recognition sites in a stretch of 500 base pairs. The crystallographic structure of the complex with DNA suggests that the tetrameric repressor functions synergistically with catabolite gene activator protein (CAP) and participates in the quaternary formation of repression loops in which one tetrameric repressor interacts simultaneously with two sites on the genomic DNA.

PubMed: 8638105

実験手法

X-RAY DIFFRACTION (3.2 Å)