1LBH

INTACT LACTOSE OPERON REPRESSOR WITH GRATUITOUS INDUCER IPTG

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1995-11-18
Detector	SIEMENS
Spacegroup name	P 21 2 21
Unit cell lengths	141.200, 75.100, 149.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 3.200
R-factor	0.23
Rwork	0.230
RMSD bond length	0.014
RMSD bond angle	1.900
Data reduction software	XDS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	15.000 *
High resolution limit [Å]	3.200 *
Rmerge	0.091
Total number of observations	110526 *
Number of reflections	26094
Completeness [%]	97.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	macro seeding *	8.5 *	22 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	PEG3000	32 (%)
2	1	drop	potassium phosphate	0.05 (M)
3	1	drop	sodium potassium tartrate	0.075 (M)
4	1	drop	protein	0.055 (mM)
5	1	drop	DNA	0.194 (mM)
6	1	reservoir	PEG3000	29-31 (%)
7	1	reservoir	potassium phosphate	0.05 (M)
8	1	reservoir	sodium potassium tartrate	0.075 (M)