1LAT

GLUCOCORTICOID RECEPTOR MUTANT/DNA COMPLEX

1LAT の概要

• エントリーDOI: 10.2210/pdb1lat/pdb
• 分子名称: DNA (5'-D(*TP*TP*CP*CP*AP*GP*AP*AP*CP*AP*TP*GP*TP*TP*CP*TP*G P*GP*A)-3'), GLUCOCORTICOID RECEPTOR, ZINC ION, ... (4 entities in total)
• 機能のキーワード: glucocorticoid receptor, dna binding regulatory protein, transcription-dna complex, transcription/dna
• 由来する生物種: Rattus norvegicus (Rat)
• 細胞内の位置: Cytoplasm (By similarity): P06536
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 30641.63

構造登録者

Gewirth, D.T.,Sigler, P.B. (登録日: 1995-12-18, 公開日: 1996-04-03, 最終更新日: 2024-02-14)

主引用文献

Gewirth, D.T.,Sigler, P.B.
The basis for half-site specificity explored through a non-cognate steroid receptor-DNA complex.
Nat.Struct.Biol., 2:386-394, 1995

PubMed Abstract: Steroid receptors recognize bipartite targets composed of six base-pair half-sites. There are two canonical types of half-site which differ only in their central two base pairs. The crystal structure of an estrogen receptor-like DNA-binding domain bound to the wrong type of half-site (a glucocorticoid response element) reveals an interface that resembles the specific interfaces of the glucocorticoid receptor or estrogen receptor bound to their correct response elements. The underlying stereochemical defect that weakens the non-cognate interface is a difference in the helical geometry of the incorrect DNA half-site which prevents a side-chain contact and results in a gap which is filled by at least five additional fixed water sites, imposing a potential entropic burden on the stability of the interface.

PubMed: 7664096
DOI: 10.1038/nsb0595-386

実験手法

X-RAY DIFFRACTION (1.9 Å)