1L3H

NMR structure of P41icf, a potent inhibitor of human cathepsin L

1L3H の概要

• エントリーDOI: 10.2210/pdb1l3h/pdb
• 関連するPDBエントリー: 1ICF
• NMR情報: BMRB: 5583
• 分子名称: MHC CLASS II-ASSOCIATED P41 INVARIANT CHAIN FRAGMENT (P41icf) (1 entity in total)
• 機能のキーワード: alpha helix, beta sheet, disulfide bonds, immune system
• 細胞内の位置: Cell membrane; Single-pass type II membrane protein (Potential): P04233
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7261.07

構造登録者

Chiva, C.,Barthe, P.,Codina, A.,Giralt, E. (登録日: 2002-02-27, 公開日: 2003-03-04, 最終更新日: 2024-10-30)

主引用文献

Chiva, C.,Barthe, P.,Codina, A.,Gairi, M.,Molina, F.,Granier, C.,Pugniere, M.,Inui, T.,Nishio, H.,Nishiuchi, Y.,Kimura, T.,Sakakibara, S.,Albericio, F.,Giralt, E.
Synthesis and NMR structure of P41ICF, a potent inhibitor of human cathepsin L
J.Am.Chem.Soc., 125:1508-1517, 2003

PubMed Abstract: The total synthesis and structural characterization of the MHCII-associated p41 invariant chain fragment (P41icf) is described. P41icf plays a crucial role in the maturation of MHC class II molecules and antigen processing, acting as a highly selective cathepsin L inhibitor. P41icf synthesis was achieved using a combined solid-phase/solution approach. The entire molecule (65 residues, 7246 Da unprotected) was assembled in solution from fully protected peptides in the size range of 10 residues. After deprotection, oxidative folding in carefully adjusted experimental conditions led to the completely folded and functional P41icf with a disulfide pairing identical to that of native P41icf. CD, NMR, and surface plasmon resonance (SPR) were used for the structural and functional characterization of synthetic P41icf. CD thermal denaturation showed clear cooperative behavior. Tight cathepsin L binding was demonstrated by SPR. (1)H NMR spectroscopy at 800 MHz of unlabeled P41icf was used to solve the three-dimensional structure of the molecule. P41icf behaves as a well-folded protein domain with a topology very close to the crystallographic cathepsin L-bound form.

PubMed: 12568610
DOI: 10.1021/ja0207908

実験手法

SOLUTION NMR