1L2X

Atomic Resolution Crystal Structure of a Viral RNA Pseudoknot

1L2X の概要

• エントリーDOI: 10.2210/pdb1l2x/pdb
• 関連するPDBエントリー: 1L3D 437D
• 分子名称: RNA pseudoknot, MAGNESIUM ION, SODIUM ION, ... (5 entities in total)
• 機能のキーワード: pseudoknot, frameshifting, atomic resolution, viral rna, metal ions, flexibility, rna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9499.38

構造登録者

Egli, M.,Minasov, G.,Su, L.,Rich, A. (登録日: 2002-02-25, 公開日: 2002-03-22, 最終更新日: 2023-08-16)

主引用文献

Egli, M.,Minasov, G.,Su, L.,Rich, A.
Metal ions and flexibility in a viral RNA pseudoknot at atomic resolution.
Proc.Natl.Acad.Sci.USA, 99:4302-4307, 2002

PubMed Abstract: Many pathogenic viruses use programmed -1 ribosomal frameshifting to regulate translation of their structural and enzymatic proteins from polycistronic mRNAs. Frameshifting is commonly stimulated by a pseudoknot located downstream from a slippery sequence, the latter positioned at the ribosomal A and P sites. We report here the structures of two crystal forms of the frameshifting RNA pseudoknot from beet western yellow virus at resolutions of 1.25 and 2.85 A. Because of the very high resolution of 1.25 A, ten mono- and divalent metal ions per asymmetric unit could be identified, giving insight into potential roles of metal ions in stabilizing the pseudoknot. A magnesium ion located at the junction of the two pseudoknot stems appears to play a crucial role in stabilizing the structure. Because the two crystal forms exhibit mostly unrelated packing interactions and local crystallographic disorder in the high-resolution form was resolvable, the two structures offer the most detailed view yet of the conformational preference and flexibility of an RNA pseudoknot.

PubMed: 11904368
DOI: 10.1073/pnas.062055599

実験手法

X-RAY DIFFRACTION (1.25 Å)