1L1C
Structure of the LicT Bacterial Antiterminator Protein in Complex with its RNA Target
Summary for 1L1C
| Entry DOI | 10.2210/pdb1l1c/pdb |
| Related | 1AUU 1H99 |
| Descriptor | licT mRNA antiterminator hairpin, Transcription antiterminator licT (2 entities in total) |
| Functional Keywords | protein rna complex, antiterminator complex, rna hairpin, transcription-rna complex, transcription/rna |
| Biological source | Bacillus subtilis More |
| Total number of polymer chains | 3 |
| Total formula weight | 21549.70 |
| Authors | Yang, Y.,Declerck, N.,Manival, X.,Aymerich, S.,Kochoyan, M. (deposition date: 2002-02-15, release date: 2002-03-27, Last modification date: 2024-05-22) |
| Primary citation | Yang, Y.,Declerck, N.,Manival, X.,Aymerich, S.,Kochoyan, M. Solution structure of the LicT-RNA antitermination complex: CAT clamping RAT. EMBO J., 21:1987-1997, 2002 Cited by PubMed Abstract: LicT is a bacterial regulatory protein able to prevent the premature arrest of transcription. When activated, LicT binds to a 29 base RNA hairpin overlapping a terminator located in the 5' mRNA leader region of the target genes. We have determined the solution structure of the LicT RNA-binding domain (CAT) in complex with its ribonucleic antiterminator (RAT) target by NMR spectroscopy (PDB 1L1C). CAT is a beta-stranded homodimer that undergoes no important conformational changes upon complex formation. It interacts, through mostly hydrophobic and stacking interactions, with the distorted minor groove of the hairpin stem that is interrupted by two asymmetric internal loops. Although different in sequence, these loops share sufficient structural analogy to be recognized similarly by symmetry-related elements of the protein dimer, leading to a quasi- symmetric structure reminiscent of that observed with dimeric transcription regulators bound to palindromic DNA. Sequence analysis suggests that this RNA- binding mode, where the RAT strands are clamped by the CAT dimer, is conserved in homologous systems. PubMed: 11953318DOI: 10.1093/emboj/21.8.1987 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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