1KXL
Solution Structure of the Cdc13 DNA-binding Domain in a Complex with Single-Stranded Telomeric DNA (DNA structure not modeled)
Summary for 1KXL
| Entry DOI | 10.2210/pdb1kxl/pdb |
| NMR Information | BMRB: 5232 |
| Descriptor | CELL DIVISION CONTROL PROTEIN 13 (1 entity in total) |
| Functional Keywords | dna-binding domain, telomere, single-stranded dna, ob fold, cell cycle |
| Biological source | Saccharomyces cerevisiae (baker's yeast) |
| Cellular location | Chromosome, telomere: P32797 |
| Total number of polymer chains | 1 |
| Total formula weight | 23575.13 |
| Authors | Mitton-Fry, R.M.,Anderson, E.M.,Wuttke, D.S. (deposition date: 2002-01-31, release date: 2002-04-10, Last modification date: 2024-05-22) |
| Primary citation | Mitton-Fry, R.M.,Anderson, E.M.,Hughes, T.R.,Lundblad, V.,Wuttke, D.S. Conserved structure for single-stranded telomeric DNA recognition. Science, 296:145-147, 2002 Cited by PubMed Abstract: The essential Cdc13 protein in the yeast Saccharomyces cerevisiae is a single-stranded telomeric DNA binding protein required for chromosome end protection and telomere replication. Here we report the solution structure of the Cdc13 DNA binding domain in complex with telomeric DNA. The structure reveals the use of a single OB (oligonucleotide/oligosaccharide binding) fold augmented by an unusually large loop for DNA recognition. This OB fold is structurally similar to OB folds found in the ciliated protozoan telomere end-binding protein, although no sequence similarity is apparent between them. The common usage of an OB fold for telomeric DNA interaction demonstrates conservation of end-protection mechanisms among eukaryotes. PubMed: 11935027DOI: 10.1126/science.1068799 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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