1KXK

Crystal Structure of a RNA Molecule Containing Domain 5 and 6 of the Yeast ai5g Group II Self-splicing Intron

Summary for 1KXK

Entry DOI	10.2210/pdb1kxk/pdb
Descriptor	ai5g group II Self-splicing intron, MAGNESIUM ION (2 entities in total)
Functional Keywords	double helix, tetraloop, tetraloop receptor, two-nucleotide bulge, rna
Total number of polymer chains	1
Total formula weight	22669.09
Authors	Zhang, L.,Doudna, J.A. (deposition date: 2002-01-31, release date: 2002-03-22, Last modification date: 2024-02-14)
Primary citation	Zhang, L.,Doudna, J.A. Structural insights into group II intron catalysis and branch-site selection. Science, 295:2084-2088, 2002 Cited by PubMed Abstract: Group II self-splicing introns catalyze autoexcision from precursor RNA transcripts by a mechanism strikingly similar to that of the spliceosome, an RNA-protein assembly responsible for splicing together the protein-coding parts of most eukaryotic pre-mRNAs. Splicing in both cases initiates via nucleophilic attack at the 5' splice site by the 2' OH of a conserved intron adenosine residue, creating a branched (lariat) intermediate. Here, we describe the crystal structure at 3.0 A resolution of a 70-nucleotide RNA containing the catalytically essential domains 5 and 6 of the yeast ai5gamma group II self-splicing intron, revealing an unexpected two-nucleotide bulged structure around the branch-point adenosine in domain 6. PubMed: 11859154 DOI: 10.1126/science.1069268 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (3 Å)

Structure validation