1KXG

The 2.0 Ang Resolution Structure of BLyS, B Lymphocyte Stimulator.

Summary for 1KXG

• Entry DOI: 10.2210/pdb1kxg/pdb
• Descriptor: B lymphocyte stimulator, MAGNESIUM ION, CITRIC ACID, ... (5 entities in total)
• Functional Keywords: baff, tall-1, thank, ztnf4, cytokine, tnf superfamily, jellyroll, beta-sandwich
• Biological source: Homo sapiens (human)
• Cellular location: Cell membrane; Single-pass type II membrane protein. Tumor necrosis factor ligand superfamily member 13b, soluble form: Secreted: Q9Y275
• Total number of polymer chains: 6
• Total formula weight: 104655.14

Authors

Oren, D.A.,Li, Y.,Volovik, Y.,Morris, T.S.,Dharia, C.,Das, K.,Galperina, O.,Gentz, R.,Arnold, E. (deposition date: 2002-01-31, release date: 2002-03-20, Last modification date: 2024-11-13)

Primary citation

Oren, D.A.,Li, Y.,Volovik, Y.,Morris, T.S.,Dharia, C.,Das, K.,Galperina, O.,Gentz, R.,Arnold, E.
Structural basis of BLyS receptor recognition.
Nat.Struct.Biol., 9:288-292, 2002

PubMed Abstract: B lymphocyte stimulator (BLyS), a member of the tumor necrosis factor (TNF) superfamily, is a cytokine that induces B-cell proliferation and immunoglobulin secretion. We have determined the three-dimensional structure of BLyS to 2.0 A resolution and identified receptor recognition segments using limited proteolysis coupled with mass spectrometry. Similar to other structurally determined TNF-like ligands, the BLyS monomer is a beta-sandwich and oligomerizes to form a homotrimer. The receptor-binding region in BLyS is a deeper, more pronounced groove than in other cytokines. The conserved elements on the 'floor' of this groove allow for cytokine recognition of several structurally related receptors, whereas variations on the 'walls' and outer rims of the groove confer receptor specificity.

PubMed: 11862220
DOI: 10.1038/nsb769

Experimental method

X-RAY DIFFRACTION (2 Å)