1KWD
SOLUTION STRUCTURE OF THE CENTRAL CONSERVED REGION OF HUMAN RESPIRATORY SYNCYTIAL VIRUS ATTACHMENT GLYCOPROTEIN G 187
Summary for 1KWD
| Entry DOI | 10.2210/pdb1kwd/pdb |
| Related | 1KWE |
| Descriptor | MAJOR SURFACE GLYCOPROTEIN G (1 entity in total) |
| Functional Keywords | cysteine nose, viral protein |
| Cellular location | Virion membrane. Isoform Secreted glycoprotein G: Secreted: P20895 |
| Total number of polymer chains | 1 |
| Total formula weight | 1741.06 |
| Authors | Sugawara, M.,Czaplicki, J.,Ferrage, J.,Haeuw, J.F.,Power, U.F.,Corvaia, N.,Nguyen, T.,Beck, A.,Milon, A. (deposition date: 2002-01-29, release date: 2003-06-17, Last modification date: 2024-11-20) |
| Primary citation | Sugawara, M.,Czaplicki, J.,Ferrage, J.,Haeuw, J.F.,Power, U.F.,Corvaia, N.,Nguyen, T.,Beck, A.,Milton, A. Structure-antigenicity relationship studies of the central conserved region of human respiratory syncytial virus protein G. J.Pept.Res., 60:271-282, 2002 Cited by PubMed Abstract: BBG2Na is a recombinant protein, composed in part of carrier protein BB and of the central conserved domain of the attachment glycoprotein G of human respiratory syncytial virus (HRSV) subgroup A. This protein is a potent vaccine candidate against HRSV. G2Na contains several contiguous B-cell epitopes, occupying sequential positions in the linear sequence of the protein. One of the epitopes contains four cysteines that are completely conserved in known strains of HRSV and form a 'cysteine noose' motif. In this study, we analysed circular dichroism (CD) spectra of BBG2Na and its B-cell epitopes. We also used NMR and molecular dynamics simulations to determine the three-dimensional structure of the cysteine noose domain. We observed significant structural differences related to the length of peptides containing the cysteine noose. These differences show good correlation with the immunogenic activity of the peptides. It is shown that a single Val(171) addition induces a pronounced structure stabilization of the cysteine noose peptide G4a (1-4/2-3) (residues 172-187), which is associated with a 100-fold increase in its antigenicity vis-à-vis a G-protein specific monoclonal antibody. PubMed: 12383117DOI: 10.1034/j.1399-3011.2002.21027.x PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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