1KSH

Complex of Arl2 and PDE delta, Crystal Form 2 (native)

1KSH の概要

• エントリーDOI: 10.2210/pdb1ksh/pdb
• 関連するPDBエントリー: 1doa 1fzq 1KSG 1KSJ
• 分子名称: arf-like protein 2, RETINAL ROD RHODOPSIN-SENSITIVE CGMP 3',5'-CYCLIC PHOSPHODIESTERASE DELTA-SUBUNIT, MAGNESIUM ION, ... (6 entities in total)
• 機能のキーワード: small gtpase, small gtp-binding protein, arf family, effector molecule, immunoglobuline-like fold, gdi, signaling protein-hydrolase complex, signaling protein/hydrolase
• 由来する生物種: Mus musculus (house mouse); 詳細
• 細胞内の位置: Mitochondrion intermembrane space: Q9D0J4
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 39333.89

構造登録者

Hanzal-Bayer, M.,Renault, L.,Roversi, P.,Wittinghofer, A.,Hillig, R.C. (登録日: 2002-01-13, 公開日: 2002-05-08, 最終更新日: 2024-11-06)

主引用文献

Hanzal-Bayer, M.,Renault, L.,Roversi, P.,Wittinghofer, A.,Hillig, R.C.
The complex of Arl2-GTP and PDE delta: from structure to function.
EMBO J., 21:2095-2106, 2002

PubMed Abstract: Arf-like (Arl) proteins are close relatives of the Arf regulators of vesicular transport, but their function is unknown. Here, we present the crystal structure of full-length Arl2-GTP in complex with its effector PDE delta solved in two crystal forms (Protein Data Bank codes 1KSG, 1KSH and 1KSJ). Arl2 shows a dramatic conformational change from the GDP-bound form, which suggests that it is reversibly membrane associated. PDE delta is structurally closely related to RhoGDI and contains a deep empty hydrophobic pocket. Further experiments show that H-Ras, Rheb, Rho6 and G alpha(i1) interact with PDE delta and that, at least for H-Ras, the intact C-terminus is required. We suggest PDE delta to be a specific soluble transport factor for certain prenylated proteins and Arl2-GTP a regulator of PDE delta-mediated transport.

PubMed: 11980706
DOI: 10.1093/emboj/21.9.2095

実験手法

X-RAY DIFFRACTION (1.8 Å)