1KQ4

CRYSTAL STRUCTURE OF A THY1-COMPLEMENTING PROTEIN (TM0449) FROM THERMOTOGA MARITIMA AT 2.25 A RESOLUTION

Summary for 1KQ4

• Entry DOI: 10.2210/pdb1kq4/pdb
• Related: 1KQ3
• Descriptor: HYPOTHETICAL PROTEIN TM0449, FLAVIN-ADENINE DINUCLEOTIDE (3 entities in total)
• Functional Keywords: thy1-complementing protein, structural genomics, joint center for structural genomics, jcsg, protein structure initiative, psi, transferase
• Biological source: Thermotoga maritima
• Total number of polymer chains: 4
• Total formula weight: 114282.40

Authors

Wilson, I.A.,Miller, M.D.,Joint Center for Structural Genomics (JCSG) (deposition date: 2002-01-03, release date: 2002-02-27, Last modification date: 2024-11-20)

Primary citation

Lesley, S.A.,Kuhn, P.,Godzik, A.,Deacon, A.M.,Mathews, I.,Kreusch, A.,Spraggon, G.,Klock, H.E.,McMullan, D.,Shin, T.,Vincent, J.,Robb, A.,Brinen, L.S.,Miller, M.D.,McPhillips, T.M.,Miller, M.A.,Scheibe, D.,Canaves, J.M.,Guda, C.,Jaroszewski, L.,Selby, T.L.,Elsliger, M.-A.,Wooley, J.,Taylor, S.S.,Hodgson, K.O.,Wilson, I.A.,Schultz, P.G.,Stevens, R.C.
Structural genomics of the Thermotoga maritima proteome implemented in a high-throughput structure determination pipeline
Proc.Natl.Acad.Sci.USA, 99:11664-11669, 2002

PubMed Abstract: Structural genomics is emerging as a principal approach to define protein structure-function relationships. To apply this approach on a genomic scale, novel methods and technologies must be developed to determine large numbers of structures. We describe the design and implementation of a high-throughput structural genomics pipeline and its application to the proteome of the thermophilic bacterium Thermotoga maritima. By using this pipeline, we successfully cloned and attempted expression of 1,376 of the predicted 1,877 genes (73%) and have identified crystallization conditions for 432 proteins, comprising 23% of the T. maritima proteome. Representative structures from TM0423 glycerol dehydrogenase and TM0449 thymidylate synthase-complementing protein are presented as examples of final outputs from the pipeline.

PubMed: 12193646
DOI: 10.1073/pnas.142413399

Experimental method

X-RAY DIFFRACTION (2.25 Å)