1KN7
Solution structure of the tandem inactivation domain (residues 1-75) of potassium channel RCK4 (Kv1.4)
Summary for 1KN7
| Entry DOI | 10.2210/pdb1kn7/pdb |
| Related | 1zto |
| NMR Information | BMRB: 5236 |
| Descriptor | VOLTAGE-GATED POTASSIUM CHANNEL PROTEIN KV1.4 (1 entity in total) |
| Functional Keywords | voltage-gated potassium channel, inactivation domain, kv1.4, rck4, membrane protein |
| Biological source | Rattus norvegicus (Norway rat) |
| Cellular location | Membrane; Multi-pass membrane protein: P15385 |
| Total number of polymer chains | 1 |
| Total formula weight | 7858.49 |
| Authors | Wissmann, R.,Bildl, W.,Oliver, D.,Beyermann, M.,Kalbitzer, H.R.,Bentrop, D.,Fakler, B. (deposition date: 2001-12-18, release date: 2003-05-06, Last modification date: 2024-05-22) |
| Primary citation | Wissmann, R.,Bildl, W.,Oliver, D.,Beyermann, M.,Kalbitzer, H.R.,Bentrop, D.,Fakler, B. Solution Structure and Function of the "Tandem Inactivation Domain" of the Neuronal A-type Potassium Channel Kv1.4 J.Biol.Chem., 278:16142-16150, 2003 Cited by PubMed Abstract: Cumulative inactivation of voltage-gated (Kv) K(+) channels shapes the presynaptic action potential and determines timing and strength of synaptic transmission. Kv1.4 channels exhibit rapid "ball-and-chain"-type inactivation gating. Different from all other Kvalpha subunits, Kv1.4 harbors two inactivation domains at its N terminus. Here we report the solution structure and function of this "tandem inactivation domain" using NMR spectroscopy and patch clamp recordings. Inactivation domain 1 (ID1, residues 1-38) consists of a flexible N terminus anchored at a 5-turn helix, whereas ID2 (residues 40-50) is a 2.5-turn helix made up of small hydrophobic amino acids. Functional analysis suggests that only ID1 may work as a pore-occluding ball domain, whereas ID2 most likely acts as a "docking domain" that attaches ID1 to the cytoplasmic face of the channel. Deletion of ID2 slows inactivation considerably and largely impairs cumulative inactivation. Together, the concerted action of ID1 and ID2 may promote rapid inactivation of Kv1.4 that is crucial for the channel function in short term plasticity. PubMed: 12590144DOI: 10.1074/jbc.M210191200 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
Download full validation report
