1KKE
Crystal Structure of Reovirus Attachment Protein Sigma1 Trimer
Summary for 1KKE
| Entry DOI | 10.2210/pdb1kke/pdb |
| Descriptor | SIGMA 1 PROTEIN (2 entities in total) |
| Functional Keywords | reovirus, sigma1, fiber, beta-spiral, beta-barrel, trimer, receptor-binding, viral protein |
| Biological source | Mammalian orthoreovirus 3 |
| Cellular location | Virion: P03528 |
| Total number of polymer chains | 3 |
| Total formula weight | 68062.24 |
| Authors | Chappell, J.D.,Prota, A.E.,Dermody, T.S.,Stehle, T. (deposition date: 2001-12-07, release date: 2001-12-21, Last modification date: 2024-02-14) |
| Primary citation | Chappell, J.D.,Prota, A.E.,Dermody, T.S.,Stehle, T. Crystal structure of reovirus attachment protein sigma1 reveals evolutionary relationship to adenovirus fiber. EMBO J., 21:1-11, 2002 Cited by PubMed Abstract: Reovirus attaches to cellular receptors with the sigma1 protein, a fiber-like molecule protruding from the 12 vertices of the icosahedral virion. The crystal structure of a receptor-binding fragment of sigma1 reveals an elongated trimer with two domains: a compact head with a new beta-barrel fold and a fibrous tail containing a triple beta-spiral. Numerous structural and functional similarities between reovirus sigma1 and the adenovirus fiber suggest an evolutionary link in the receptor-binding strategies of these two viruses. A prominent loop in the sigma1 head contains a cluster of residues that are conserved among reovirus serotypes and are likely to form a binding site for junction adhesion molecule, an integral tight junction protein that serves as a reovirus receptor. The fibrous tail is mainly responsible for sigma1 trimer formation, and it contains a highly flexible region that allows for significant movement between the base of the tail and the head. The architecture of the trimer interface and the observed flexibility indicate that sigma1 is a metastable structure poised to undergo conformational changes upon viral attachment and cell entry. PubMed: 11782420DOI: 10.1093/emboj/21.1.1 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.6 Å) |
Structure validation
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