1KHV

Crystal Structure of Rabbit Hemorrhagic Disease Virus RNA-dependent RNA polymerase complexed with Lu3+

1KHV の概要

• エントリーDOI: 10.2210/pdb1khv/pdb
• 関連するPDBエントリー: 1khw
• 分子名称: RNA-DIRECTED RNA POLYMERASE, LUTETIUM (III) ION (3 entities in total)
• 機能のキーワード: rna-dependent rna polymerase, transferase
• 由来する生物種: Rabbit hemorrhagic disease virus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 116012.68

構造登録者

Ng, K.K.,Cherney, M.M.,Vazquez, A.L.,Machin, A.,Alonso, J.M.,Parra, F.,James, M.N. (登録日: 2001-12-01, 公開日: 2002-01-16, 最終更新日: 2024-02-14)

主引用文献

Ng, K.K.,Cherney, M.M.,Vazquez, A.L.,Machin, A.,Alonso, J.M.,Parra, F.,James, M.N.
Crystal structures of active and inactive conformations of a caliciviral RNA-dependent RNA polymerase.
J.Biol.Chem., 277:1381-1387, 2002

PubMed Abstract: The structure of the RNA-dependent RNA polymerase (RdRP) from the rabbit hemorrhagic disease virus has been determined by x-ray crystallography to a 2.5-A resolution. The overall structure resembles a "right hand," as seen before in other polymerases, including the RdRPs of polio virus and hepatitis C virus. Two copies of the polymerase are present in the asymmetric unit of the crystal, revealing active and inactive conformations within the same crystal form. The fingers and palm domains form a relatively rigid unit, but the thumb domain can adopt either "closed" or "open" conformations differing by a rigid body rotation of approximately 8 degrees. Metal ions bind at different positions in the two conformations and suggest how structural changes may be important to enzymatic function in RdRPs. Comparisons between the structures of the alternate conformational states of rabbit hemorrhagic disease virus RdRP and the structures of RdRPs from hepatitis C virus and polio virus suggest novel structure-function relationships in this medically important class of enzymes.

PubMed: 11677245
DOI: 10.1074/jbc.M109261200

実験手法

X-RAY DIFFRACTION (2.5 Å)