1KHV

Crystal Structure of Rabbit Hemorrhagic Disease Virus RNA-dependent RNA polymerase complexed with Lu3+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	1999-10-05
Detector	ADSC QUANTUM 4
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	70.300, 119.100, 158.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.500 - 2.500
R-factor	0.218 *
Rwork	0.217
R-free	0.26800 *
Structure solution method	MAD
RMSD bond length	0.007 *
RMSD bond angle	1.190 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000 *	2.640
High resolution limit [Å]	2.500	2.500
Rmerge	0.039	0.254
Total number of observations	125565 *
Number of reflections	42943
<I/σ(I)>	13.6	2.7
Completeness [%]	91.4	82.4
Redundancy	2.9	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	PEG 8000, potassium thiocyanate, L-proline, glycerol, hexanediol, CHAPS, lutetium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	PEG8000	10-12 (%)
3	1	reservoir	Tris-Cl	0.1 (M)	pH7.5
4	1	reservoir	sodium thiocyanate	0.2 (M)
5	1	reservoir	L-proline	0.1 (M)
6	1	reservoir	glycerol	15 (%(w/v))
7	1	reservoir	1,6-hexanediol	7 (%(v/v))
8	1	reservoir	CHAPS	0.1 (%(w/v))
9	1	reservoir		5 (mM)