1KHV
Crystal Structure of Rabbit Hemorrhagic Disease Virus RNA-dependent RNA polymerase complexed with Lu3+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-10-05 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 70.300, 119.100, 158.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.500 - 2.500 |
R-factor | 0.218 * |
Rwork | 0.217 |
R-free | 0.26800 * |
Structure solution method | MAD |
RMSD bond length | 0.007 * |
RMSD bond angle | 1.190 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 * | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.039 | 0.254 |
Total number of observations | 125565 * | |
Number of reflections | 42943 | |
<I/σ(I)> | 13.6 | 2.7 |
Completeness [%] | 91.4 | 82.4 |
Redundancy | 2.9 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | PEG 8000, potassium thiocyanate, L-proline, glycerol, hexanediol, CHAPS, lutetium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15 (mg/ml) | |
2 | 1 | reservoir | PEG8000 | 10-12 (%) | |
3 | 1 | reservoir | Tris-Cl | 0.1 (M) | pH7.5 |
4 | 1 | reservoir | sodium thiocyanate | 0.2 (M) | |
5 | 1 | reservoir | L-proline | 0.1 (M) | |
6 | 1 | reservoir | glycerol | 15 (%(w/v)) | |
7 | 1 | reservoir | 1,6-hexanediol | 7 (%(v/v)) | |
8 | 1 | reservoir | CHAPS | 0.1 (%(w/v)) | |
9 | 1 | reservoir | 5 (mM) |