1K9A

Crystal structure analysis of full-length carboxyl-terminal Src kinase at 2.5 A resolution

1K9A の概要

• エントリーDOI: 10.2210/pdb1k9a/pdb
• 関連するPDBエントリー: 1AD5 1BYG 1CSK 1FMK 1JWO 3LCK
• 分子名称: Carboxyl-terminal Src kinase (2 entities in total)
• 機能のキーワード: carboxyl-terminal src kinase, cooh-terminal src kinase, csk, src, sfk, signal transduction, sh2, sh3, src homology, tyrosine kinase, cytoplasmic tyrosine kinase, cbp, oncogene, cancer, transferase
• 由来する生物種: Rattus norvegicus (Norway rat)
• 細胞内の位置: Cytoplasm (By similarity): P32577
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 304879.31

構造登録者

Ogawa, A.,Takayama, Y.,Nagata, A.,Chong, K.T.,Takeuchi, S.,Sakai, H.,Nakagawa, A.,Nada, S.,Okada, M.,Tsukihara, T. (登録日: 2001-10-28, 公開日: 2002-03-20, 最終更新日: 2024-11-20)

主引用文献

Ogawa, A.,Takayama, Y.,Sakai, H.,Chong, K.T.,Takeuchi, S.,Nakagawa, A.,Nada, S.,Okada, M.,Tsukihara, T.
Structure of the carboxyl-terminal Src kinase, Csk.
J.Biol.Chem., 277:14351-14354, 2002

PubMed Abstract: The carboxyl-terminal Src kinase (Csk) is an indispensable negative regulator for the Src family tyrosine kinases (SFKs) that play pivotal roles in various cell signalings. To understand the molecular basis of the Csk-mediated regulation of SFKs, we elucidated the crystal structure of full-length Csk. The Csk crystal consists of six molecules classified as active or inactive states according to the coordinations of catalytic residues. Csk assembles the SH2 and SH3 domains differently from inactive SFKs, and their binding pockets are oriented outward enabling the intermolecular interaction. In active molecules, the SH2-kinase and SH2-SH3 linkers are tightly stuck to the N-lobe of the kinase domain to stabilize the active conformation, and there is a direct linkage between the SH2 and the kinase domains. In inactive molecules, the SH2 domains are rotated destroying the linkage to the kinase domain. Cross-correlation matrices for the active molecules reveal that the SH2 domain and the N-lobe of the kinase domain move as a unit. These observations suggest that Csk can be regulated through coupling of the SH2 and kinase domains and that Csk provides a novel built-in activation mechanism for cytoplasmic tyrosine kinases.

PubMed: 11884384
DOI: 10.1074/jbc.C200086200

実験手法

X-RAY DIFFRACTION (2.5 Å)