1K7B

NMR Solution Structure of sTva47, the Viral-Binding Domain of Tva

Summary for 1K7B

• Entry DOI: 10.2210/pdb1k7b/pdb
• NMR Information: BMRB: 5210
• Descriptor: SUBGROUP A ROUS SARCOMA VIRUS RECEPTOR PG800 AND PG950 (1 entity in total)
• Functional Keywords: beta hairpin, 3-10 helix, calcium binding, membrane protein
• Biological source: Coturnix coturnix (common quail)
• Total number of polymer chains: 1
• Total formula weight: 5118.46

Authors

Tonelli, M.,Peters, R.J.,James, T.L.,Agard, D.A. (deposition date: 2001-10-18, release date: 2001-12-19, Last modification date: 2024-11-20)

Primary citation

Tonelli, M.,Peters, R.J.,James, T.L.,Agard, D.A.
The solution structure of the viral binding domain of Tva, the cellular receptor for subgroup A avian leukosis and sarcoma virus.
FEBS Lett., 509:161-168, 2001

PubMed Abstract: The cellular receptor for subgroup A avian leukosis and sarcoma virus (ALSV-A) is Tva, which contains a motif related to repeats in the low density lipoprotein receptor (LDLR) ligand binding repeat (LBr) and which is necessary for viral entry. As observed with LBr repeats of LDLR, the 47 residue LBr domain of Tva (sTva47) requires calcium during oxidative folding to form the correct disulfide bonds, and calcium enhances the structure of correctly oxidized sTva47, as well as its ability to bind the viral envelope protein (Env). However, solution nuclear magnetic resonance studies indicate that, even in the presence of excess calcium, sTva47 exists in an ensemble of conformations. Nonetheless, as reported here, the structure of the predominant sTva47 solution conformer closely resembles that of other LBr repeats, with identical S-S binding topology and octahedral calcium coordination. The location of W48 and other critical residues on the surface suggests a region of the molecule necessary for Env binding and to mediate post-binding events important for ALSV-A cell entry.

PubMed: 11768384
DOI: 10.1016/S0014-5793(01)03086-1

Experimental method

SOLUTION NMR