1K78

Pax5(1-149)+Ets-1(331-440)+DNA

Summary for 1K78

• Entry DOI: 10.2210/pdb1k78/pdb
• Related: 1K79 1K7A
• Descriptor: Pax5/Ets Binding Site on the mb-1 promoter, Paired Box Protein Pax5, C-ets-1 Protein, ... (5 entities in total)
• Functional Keywords: paired domain, ets domain, transcription factor, transcription-dna complex, transcription/dna
• Biological source: Homo sapiens (human); More
• Cellular location: Nucleus: Q02548 P27577
• Total number of polymer chains: 9
• Total formula weight: 109126.50

Authors

Garvie, C.W.,Hagman, J.,Wolberger, C. (deposition date: 2001-10-18, release date: 2002-01-04, Last modification date: 2024-02-07)

Primary citation

Garvie, C.W.,Hagman, J.,Wolberger, C.
Structural studies of Ets-1/Pax5 complex formation on DNA.
Mol.Cell, 8:1267-1276, 2001

PubMed Abstract: Pax5 regulates the B cell-specific expression of the mb-1 gene together with members of the Ets family of transcriptional activators. The Ets proteins on their own bind poorly to the Pax5/Ets binding site, but can be recruited to the site by cooperative interactions with Pax5. The structure of the ETS domain of Ets-1 and the paired domain of Pax5 bound to DNA reveals the molecular details of the selective recruitment of different Ets proteins by Pax5. Comparison with structures of Ets-1 alone bound to both high- and low-affinity DNA sites reveals that Pax5 alters the Ets-1 contacts with DNA. The ability of one protein to alter the DNA sequence-specific contacts of another provides a general mechanism for combinatorial regulation of transcription.

PubMed: 11779502
DOI: 10.1016/S1097-2765(01)00410-5

Experimental method

X-RAY DIFFRACTION (2.25 Å)