1K2W

Crystal structure of sorbitol dehydrogenase from R. sphaeroides

Summary for 1K2W

• Entry DOI: 10.2210/pdb1k2w/pdb
• Descriptor: SORBITOL DEHYDROGENASE (2 entities in total)
• Functional Keywords: short-chain dehydrogenase, enzyme, oxidoreductase
• Biological source: Rhodobacter sphaeroides
• Total number of polymer chains: 2
• Total formula weight: 54081.68

Authors

Philippsen, A.,Schirmer, T.,Stetefeld, J. (deposition date: 2001-09-29, release date: 2003-11-11, Last modification date: 2023-08-16)

Primary citation

Philippsen, A.,Schirmer, T.,Stein, M.A.,Giffhorn, F.,Stetefeld, J.
Structure of zinc-independent sorbitol dehydrogenase from Rhodobacter sphaeroides at 2.4 A resolution.
Acta Crystallogr.,Sect.D, 61:374-379, 2005

PubMed Abstract: Recombinant sorbitol dehydrogenase (SDH) from Rhodobacter sphaeroides has been crystallized in the absence of the cofactor NAD(H) and its structure determined to 2.4 A resolution using molecular replacement (refined R and R free factors of 18.8 and 23.8%, respectively). As expected from the sequence and shown by the conserved fold, SDH can be assigned to the short-chain dehydrogenase/reductase protein family. The cofactor NAD and the substrate sorbitol have been modelled into the structure and the active-site architecture, which displays the highly conserved catalytic tetrad of Asn-Ser-Tyr-Lys residues, is discussed in relation to the enzyme mechanism. This is the first structure of a bacterial SDH belonging to the SDR family.

PubMed: 15805591
DOI: 10.1107/S0907444904034390

Experimental method

X-RAY DIFFRACTION (2.4 Å)