1K1S
Crystal Structure of DinB from Sulfolobus solfataricus
Summary for 1K1S
| Entry DOI | 10.2210/pdb1k1s/pdb |
| Related | 1K1Q |
| Descriptor | DBH protein (2 entities in total) |
| Functional Keywords | mixed a/b structure, 4-stranded antiparallel b-sheet, transcription |
| Biological source | Sulfolobus solfataricus |
| Cellular location | Cytoplasm (Probable): P96022 |
| Total number of polymer chains | 1 |
| Total formula weight | 40029.77 |
| Authors | Silvian, L.F.,Toth, E.A.,Pham, P.,Goodman, M.F.,Ellenberger, T. (deposition date: 2001-09-25, release date: 2001-10-31, Last modification date: 2023-08-16) |
| Primary citation | Silvian, L.F.,Toth, E.A.,Pham, P.,Goodman, M.F.,Ellenberger, T. Crystal structure of a DinB family error-prone DNA polymerase from Sulfolobus solfataricus. Nat.Struct.Biol., 8:984-989, 2001 Cited by PubMed Abstract: A new group of error-prone DNA polymerases overcomes the blockage posed to normal DNA replication by damaged template bases, suggesting an active site with a loose, flexible pocket that accommodates aberrant DNA structures. We have determined a 2.8 A resolution crystal structure of the Sulfolobus solfataricus Dbh protein, a DNA translesion polymerase closely related to Escherichia coli DNA polymerase IV and human polymerase kappa. A high error rate is observed for the Dbh polymerase in a range of 10(-2)-10(-3) for all 12 base substitution mispairs. The crystal structure of Dbh reveals an overall architecture resembling other DNA polymerases but has unique features that are likely to contribute to error-prone synthesis, including -1 frameshifting mutations. PubMed: 11685247DOI: 10.1038/nsb1101-984 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.8 Å) |
Structure validation
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