1K1S

Crystal Structure of DinB from Sulfolobus solfataricus

1K1S の概要

• エントリーDOI: 10.2210/pdb1k1s/pdb
• 関連するPDBエントリー: 1K1Q
• 分子名称: DBH protein (2 entities in total)
• 機能のキーワード: mixed a/b structure, 4-stranded antiparallel b-sheet, transcription
• 由来する生物種: Sulfolobus solfataricus
• 細胞内の位置: Cytoplasm (Probable): P96022
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 40029.77

構造登録者

Silvian, L.F.,Toth, E.A.,Pham, P.,Goodman, M.F.,Ellenberger, T. (登録日: 2001-09-25, 公開日: 2001-10-31, 最終更新日: 2023-08-16)

主引用文献

Silvian, L.F.,Toth, E.A.,Pham, P.,Goodman, M.F.,Ellenberger, T.
Crystal structure of a DinB family error-prone DNA polymerase from Sulfolobus solfataricus.
Nat.Struct.Biol., 8:984-989, 2001

PubMed Abstract: A new group of error-prone DNA polymerases overcomes the blockage posed to normal DNA replication by damaged template bases, suggesting an active site with a loose, flexible pocket that accommodates aberrant DNA structures. We have determined a 2.8 A resolution crystal structure of the Sulfolobus solfataricus Dbh protein, a DNA translesion polymerase closely related to Escherichia coli DNA polymerase IV and human polymerase kappa. A high error rate is observed for the Dbh polymerase in a range of 10(-2)-10(-3) for all 12 base substitution mispairs. The crystal structure of Dbh reveals an overall architecture resembling other DNA polymerases but has unique features that are likely to contribute to error-prone synthesis, including -1 frameshifting mutations.

PubMed: 11685247
DOI: 10.1038/nsb1101-984

実験手法

X-RAY DIFFRACTION (2.8 Å)