1K1G

STRUCTURAL BASIS FOR RECOGNITION OF THE INTRON BRANCH SITE RNA BY SPLICING FACTOR 1

Summary for 1K1G

• Entry DOI: 10.2210/pdb1k1g/pdb
• Descriptor: 5'-R(*UP*AP*UP*AP*CP*UP*AP*AP*CP*AP*A)-3', SF1-Bo isoform (2 entities in total)
• Functional Keywords: splicing, branch point sequence, protein/rna recognition, complex e, kh domain, qua2 homology, star proteins, gene regulation-rna complex, gene regulation/rna
• Biological source: Homo sapiens (human)
• Cellular location: Nucleus: Q15637
• Total number of polymer chains: 2
• Total formula weight: 18162.03

Authors

Liu, Z.,Luyten, I.,Bottomley, M.J.,Messias, A.C.,Houngninou-Molango, S.,Sprangers, R.,Zanier, K.,Kramer, A.,Sattler, M. (deposition date: 2001-09-25, release date: 2001-11-07, Last modification date: 2024-05-22)

Primary citation

Liu, Z.,Luyten, I.,Bottomley, M.J.,Messias, A.C.,Houngninou-Molango, S.,Sprangers, R.,Zanier, K.,Kramer, A.,Sattler, M.
Structural basis for recognition of the intron branch site RNA by splicing factor 1.
Science, 294:1098-1102, 2001

PubMed Abstract: During spliceosome assembly, splicing factor 1 (SF1) specifically recognizes the intron branch point sequence (BPS) UACUAAC in the pre-mRNA transcripts. We show that the KH-QUA2 region of SF1 defines an enlarged KH (hn RNP K) fold which is necessary and sufficient for BPS binding. The 3' part of the BPS (UAAC), including the conserved branch point adenosine (underlined), is specifically recognized in a hydrophobic cleft formed by the Gly-Pro-Arg-Gly motif and the variable loop of the KH domain. The QUA2 region recognizes the 5' nucleotides of the BPS (ACU). The branch point adenosine acting as the nucleophile in the first biochemical step of splicing is deeply buried. BPS RNA recognition suggests how SF1 may facilitate subsequent formation of the prespliceosomal complex A.

PubMed: 11691992
DOI: 10.1126/science.1064719

Experimental method

SOLUTION NMR